What are the commonly used detection methods for plant extracts?
First of all, there are different methods for different detection of plant extracts. The more commonly used detection methods are HPLC, UV, TLC, GC and AAS. I believe that many readers do not understand very well, so today Xiaobian will explain their respective detections to you. specific content and methods.
HPLC and UV are common detection methods for standard plant extracts
TLC was used for the detection of ratio extracts
GC is used to detect volatile liquids or oils
AAS is used for the detection of heavy metal content in extracts
1. High Performance Liquid Chromatography (HPLC)
High-performance liquid chromatography uses liquid as the mobile phase and uses a high-pressure infusion system to pump a single solvent with different polarities or a mixed solvent of different proportions, buffers and other mobile phases into a chromatographic column equipped with a stationary phase. After the components are separated, they enter the detector for detection, thereby realizing the analysis of the sample. This method has become an important separation and analysis technology in the fields of chemistry, medicine, industry, agriculture, commodity inspection and legal inspection.
2. Ultraviolet absorption spectrometry (UV)
UV detection method utilizes the molecular absorption of certain substances to absorb radiation in the 10-800nm spectral region for analysis and determination, and is widely used in the qualitative and quantitative determination of organic and inorganic substances. The method has the characteristics of high sensitivity, good accuracy, excellent selectivity, easy operation and good analysis speed. The UV absorption spectrum of a substance is basically the characteristics of the chromophore and auxochromophore in its molecule, rather than the characteristics of the whole molecule.
3. Thin Layer Chromatography (TLC) Apply a suitable stationary phase to a glass plate, plastic or aluminum substrate to form a uniform thin layer. After sampling and development, compare the ratio shift value (Rf) with the ratio shift value (Rf) of the chromatogram obtained by the same method according to the appropriate reference substance, which is used for the identification of drugs, the method for impurity inspection or content determination . Thin-layer chromatography is an important experimental technique for rapid separation and qualitative analysis of small amounts of substances
4. Gas chromatography (GC) Chromatography using gas as the mobile phase.
5. Atomic Absorption Spectroscopy (AAS) is an analytical method based on the quantification of the measured element content based on the absorption intensity of the outer electrons of the gaseous ground state atoms to the corresponding atomic resonance radiation in the ultraviolet and visible ranges. Analysis of trace and trace components in samples.
